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KMID : 1044520170800010060
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Tuberculosis and Respiratory Diseases
2017 Volume.80 No. 1 p.60 ~ p.68
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Mometasone Furoate Suppresses PMA-Induced MUC-5AC and MUC-2 Production in Human Airway Epithelial Cells
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Poachanukoon Orapan
Koontongkaew Sittichai
Monthanapisut Paopanga
Pattanacharoenchai Napaporn
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Abstract
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Background: Mucus hypersecretion from airway epithelium is a characteristic feature of airway inflammatory diseases. Tumor necrosis factor α (TNF-α) regulates mucin synthesis. Glucocorticoids including mometasone fuorate (MF) have been used to attenuate airway inflammation. However, effects of MF on mucin production have not been reported.
Methods: Effects of MF and budesonide (BUD) on the phorbol-12-myristate-13-acetate (PMA)?induction of mucin and TNF-α in human airway epithelial cells (NCI-H292) were investigated in the present study. Confluent NCI-H292 cells were pretreated with PMA (200 nM) for 2 hours. Subsequently, the cells were stimulated with MF (1?500 ng/mL) or BUD (21.5 ng/mL) for 8 hours. Dexamethasone (1 μg/mL) was used as the positive control. Real-time polymerase chain reaction was used to determine MUC2 and MUC5AC mRNA levels. The level of total mucin, MUC2, MUC5AC, and TNF-α in culture supernatants were measured using enzyme-linked immunosorbent assay.
Results: MF and BUD significantly suppressed MUC2 and MUC5AC gene expression in PMA-stimulated NCI-H292 cells. The inhibitory effects of the two steroid drugs were also observed in the production of total mucin, MUC2 and MUC5AC proteins, and TNF-α.
Conclusion: Our findings demonstrated that MF and BUD attenuated mucin and TNF-α production in PMA-induced human airway epithelial cells.
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KEYWORD
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Mometasone Fuorate, Budesonide, Mucins, MUC2 Protein, Human, MUC5AC Protein, Human, Tumor Necrosis Factor-Alpha, Epithelial Cells
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